Effects of ouabain on insulin secretion in the dog.

نویسندگان

  • L Triner
  • J Papayoanou
  • P Killian
  • Y Vulliemoz
  • R Castany
  • G G Nahas
چکیده

The mechanism of the previously reported hypoglycemic effect of ouabain was studied in dogs. Ouabain (1.0 /xg/kg/min infused intravenously for 60 minutes) combined with insulin or propranolol caused a greater and more prolonged decrease in blood glucose than either of the drugs administered separately. In intact dogs, ouabain changed significantly portohepatic venous differences in plasma glucose from +6.6 to —13.6 mg/100 ml and in K from —0.03 to —0.4 mEq/liter. In pancreatectomized animals, ouabain did not cause any significant decrease in peripheral glucose level, and the portohepatic differences in plasma glucose changed from +5.3 to +30.2 mg/100 ml and the differences in K from —0.01 to +0.2 mEq/liter. These changes, indicating an increased release of glucose and K by the liver in the pancreatectomized dog, were also observed in isolated rat liver perfused with ouabain 10~M. Glucose uptake of the hindlimb increased significantly during the infusion of ouabain in normal dogs, but did not change in pancreatectomized dogs. Ouabain caused a significant increase in plasma insulin in portal blood ( + 155%). These results demonstrate that the observed metabolic effects of ouabain in the dog are mainly mediated by insulin and that ouabain increases the secretion of insulin in intact dogs. ADDITIONAL

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عنوان ژورنال:
  • Circulation research

دوره 25 2  شماره 

صفحات  -

تاریخ انتشار 1969